TCF-1 Promotes Chromatin Interactions across Topologically Associating Domains in T Cell Progenitors

^{*Co-first authors}

The high mobility group (HMG) transcription factor TCF-1 is essential for early T cell development. Using gain- and loss-of-function experiments, we discovered that the co-occupancy of TCF-1 and the architectural protein CTCF altered the structure of topologically associating domains (TADs) in T cell progenitors, leading to interactions between previously insulated regulatory elements and target genes at late stages of T cell development. The TCF-1-dependent gain in long-range interactions was linked to deposition of the active enhancer mark H3K27ac and recruitment of the cohesin-loading factor NIPBL at active enhancers.

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Recommended citation: Wang W^*, Chandra A^*, Goldman N^*, Yoon S^*, Ferrari EK^*, Nguyen SC, Joyce EF, Vahedi G. (2022). "TCF-1 promotes chromatin interactions across topologically associating domains in T cell progenitors." Nature Immunology. 23(7):1052–1062. doi:10.1038/s41590-022-01232-z
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