Joint Profiling of Chromatin Accessibility and CAR-T Integration Site Analysis at Population and Single-Cell Levels
Published in Proceedings of the National Academy of Sciences, 2020
Chimeric antigen receptor (CAR)-T immunotherapy has yielded impressive results in several B cell malignancies. A major outstanding question is whether CAR-T integration enhances the proliferative competence of individual T cells by rewiring their regulatory landscape. We developed EpiVIA (GitHub) for the joint profiling of chromatin accessibility and lentiviral integration sites at population and single-cell levels, enabling single-cell deconstruction of gene regulation during CAR-T therapy.
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Recommended citation: Wang W, Fasolino M, Cattau B, Goldman N, Kong W, Frederick MA, McCright SJ, Kiani K, Fraietta JA, Vahedi G. (2020). "Joint profiling of chromatin accessibility and CAR-T integration site analysis at population and single-cell levels." Proceedings of the National Academy of Sciences. 117(10):5442–5452. doi:10.1073/pnas.1919259117
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